Abstract：The FT-IR spectra of the four types of pine needles (red pine, pinus taiwanensis, masson pine, cedar) were firstly obtained by Fourier transform infrared spectrometry. Through the analysis of the spectra, we can conclude that the spectra of the four types of pine needles are very similar. But the wave-numbers and the height of the absorption peaks, especially those at the fingerprint zone (1382 ～ 1255 cm- 1, 1050 ~ 607 cm- 1) are the characteristics of each of the species. By the degree of similarity of the IR spectra, it could be concluded that red pine and masson pine, as well as pinus taiwanensis and cedar are closely related. According to the intensity and the location of these absorption peaks in IR spectra, we can infer that pine needles may contain chemical compositions such as polysaccharide, amino acid, alkane, alkaloids, triterpene compounds, stigmasterol, lignin, cellulose and volatile oil. And these chemical constituents in pinus taiwanensis and cedar are more than that in red pine and pinus massoniana. For the four kinds of pine needles, the chemical composition of pinus taiwanensis pine needles is the most, and the chemical composition of masson pine needles is the least. The results will provide scientific evidences for the identification, development and the utilization of pines.