基于关节软骨组织中胶原蛋白的自发荧光特性，对软骨组织的荧光显微检测方法进行研究。首先基于荧光图像的统计处理，揭示健康样本与病变样本的差异。其次基于荧光的偏振敏感特性，利用线性二色性方法揭示了软骨组织存在的荧光各向异性特性，为利用荧光各向异性方法研究生物组织结构奠定基础。与传统的显微方法检测软骨组织的形态和超结构相比，利用荧光特性的荧光显微检测方法具有获取软骨组织结构的能力。本文的研究结果为开辟新的骨关节疾病诊断方法提供基础。

   Based on the auto-fluorescence property of collagen in articular cartilage, the research on fluorescence microscopy measurement method of articular cartilage was carried out. First, the difference between the healthy and pathological samples was revealed by statistical comparison of fluorescence images. Second, based on the polarization property of fluorescence, the fluorescence anisotropy of the cartilage was presented by the linear dichroism method, which lays foundation for research on structure of articular cartilage using fluorescence anisotropy method. Compared to traditional microscopic measurement techniques that supply the morphology and the super-structure of cartilage tissues, fluorescence microscopy measurement methods are able to obtain the information about the structure of cartilage tissues. The results presented in this paper open the door for developing new diagnostic method for articular cartilage diseases.