Raman microscopy was applied to analyze pigments samples were collected from murals on the pilgrimage-corridor walls at Jokhang Temple,Lhasa,Tibet,China. The results show that red pigments are cinnabar, red lead and toluidine red,yellow pigments are orpiment, lead chrome yellow and iron (III) oxide monohydrate, blue pigments are azurite and ultramarine blue, green pigments are malachite, brochantite and emerald green, write pigments are magnesite, ithopone and calcite. Synthetic organic pigments(Toluidine Red )was founded firse time. The investigation can provide accurate and reliable information for the clear understanding different historical periods mural painting and restoration pigments.