建立窄带薄层色谱-表面增强拉曼光谱（narrow-band TLC-SERS）分析方法来改善传统薄层色谱-表面增强拉曼光谱法（TLC-SERS）在色谱展开过程中斑点横向扩散以及由于滴加SERS基底而导致的斑点二次扩散的不足，进一步提高TLC-SERS方法的检测灵敏度。本文采用色谱层析硅胶GF254制备并优化出宽度为2 mm的窄带TLC板，待样品在窄带TLC板上分离后，在分离斑点表面喷洒银溶胶，然后对斑点进行SERS检测。结果表明，narrow-band TLC-SERS法具有简便、快速、灵敏的特点，不仅改善了传统TLC-SERS方法斑点横向扩散的不足，而且降低了固定相的用量，节约了成本，具有非常广阔的应用前景。

The narrow-band thin layer chromatography combing with surface enhanced Raman spectroscopy (narrow-band TLC-SERS) method was established to further improve the sensitivity of conventional TLC-SERS method, which can restrain transverse diffusion of analytes in TLC development and overcame the second diffusion of analytes attributed to adding SERS-active substrate. In this work, the narrow-band TLC plate with width of 2 mm was prepared using the silica gel GF254 as stationary phase. After the sample applied on the narrow-band TLC plate, the silver colloid was deposited on the surface of separated spots, and then the spots were detected by portable Raman spectrometer. The results indicate that the narrow-band TLC-SERS method is simple, rapid and sensitive. Furthermore, this method not only restrains the defect of the transverse diffusion of analytes in TLC development but also saving the cost. Thus, the narrow-band TLC-SERS method has good prospects for application.