人工老化和自然老化大豆种子的红外光谱研究

刘杰, 刘刚, 李姝洁, 邓子昂, 欧全宏, 时有明

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光散射学报 ›› 2021, Vol. 33 ›› Issue (1) : 84-92. DOI: 10.13883/j.issn1004-5929.202101012
其它光谱技术及应用

人工老化和自然老化大豆种子的红外光谱研究

  • 刘杰1, 刘刚1*, 李姝洁1, 邓子昂1, 欧全宏1, 时有明2
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Infrared Spectra of Artificial Aging and Natural Aging of Soybean Seeds

  • LIU Jie1, LIU Gang1*, LI Shujie1, DENG Ziang1, OU Quanhong1, SHI Youming2
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摘要

利用傅里叶变换红外光谱、二阶导数红外光谱结合二维相关红外光谱法,研究了人工老化和自然老化条件下的大豆种子。结果显示,两种老化条件下大豆种子的原始光谱整体相似,吸收峰强度出现了差异;在二阶导数红外光谱中,人工老化和自然老化条件下大豆种子中物质变化趋势趋于一致。两种老化大豆种子在1747和1693 cm-1附近的吸光度随着老化程度的增加而增强的趋势;在1660,1549,1236 cm-1附近整体为减弱趋势;在1610~1550 cm-1附近呈现增加趋势;在1150~1100 cm-1附近呈现减弱趋势,但在老化过程中均出现一定增强。二维相关红外光谱结果显示,未经老化处理的大豆种子的自动峰数目和位置相同,自动峰强度发生改变;自然老化条件下大豆种子随着老化程度的加深,自动峰的数量、强度和位置表现出明显差异;人工老化大豆种子随着老化程度的加深,自动峰的数目和位置差异不明显,强度变化较为明显。大豆种子在老化过程中脂类物质增加,蛋白质物质减少,糖类物质减少,酮/醛类物质增加,氨基酸增加,醇类物质、酚类物质发生变化。利用红外光谱法对人工老化和自然老化条件下大豆种子的研究结果表明,红外光谱法可以实现快速、方便的对两种老化大豆种子的光谱变化进行研究。

Abstract

In this paper, soybean seeds under artificial aging and natural aging conditions were studied by infrared spectroscopy. The results showed that the original spectra of soybean seeds under the two aging conditions were similar, and there were differences in the intensity of absorption peaks. In the second derivative infrared spectra, the change trend of substances in soybean seeds under artificial aging and natural aging conditions tends to be consistent. The absorption peak intensity of two kinds of aging soybean seeds increased with the increase of aging degree around 1747 and 1693 cm-1. The intensity of absorption peaks around 1660, 1549, and 1236 cm-1 showed a weakening trend overall. In the vicinity of 1610-1550 cm-1, the absorption peak intensity shows an increasing trend. The intensity of the absorption peak near 1150-1100 cm-1 shows a weakening trend, but there is a certain increase in the aging process. The results of two-dimensional correlation infrared spectroscopy showed that the number and position of auto-peaks of unaged soybean seeds were the same, and the auto-peaks intensity changed. Under natural aging conditions, the number, intensity and location of auto-peaks of soybean seeds show obvious differences as the degree of aging deepens. As the aging degree of artificially aged soybean seeds deepens, the number and position of auto-peaks are not significantly different, and the intensity changes are more obvious. Lipid substances, amino acids and ketone/aldehyde substances all show an increasing trend during the aging process of soybean seeds, protein substances and sugar substances show a decreasing trend, alcohol substances and phenol substances change.The results of using infrared spectroscopy on soybean seeds under artificial aging and natural aging conditions show that infrared spectroscopy can quickly and conveniently study the spectral changes of two kinds of aging soybean seeds.

关键词

大豆种子 / 人工老化 / 自然老化 / 红外光谱

Key words

soybean seeds / artificial aging / natural aging / infrared spectroscopy

引用本文

导出引用
刘杰, 刘刚, 李姝洁, 邓子昂, 欧全宏, 时有明. 人工老化和自然老化大豆种子的红外光谱研究. 光散射学报. 2021, 33(1): 84-92 https://doi.org/10.13883/j.issn1004-5929.202101012
LIU Jie, LIU Gang, LI Shujie, DENG Ziang, OU Quanhong, SHI Youming. Infrared Spectra of Artificial Aging and Natural Aging of Soybean Seeds. Chinese Journal of Light Scattering. 2021, 33(1): 84-92 https://doi.org/10.13883/j.issn1004-5929.202101012

参考文献

[1]王仪春, 王洋, 陆敏, 等. 人工老化处理对不同休眠特性水稻种子生理特性的影响[J]. 种子, 2018, 37(6): 15-19.(WANG Yichun, WANG Yang, LU Min, WU Hongkai, et al. Effects of Artificial Aging on Physiological Characteristics of Rice Seeds in Different Dormancy Properties[J]. Seed, 2018, 37(6):15-19.)
[2]Xin X, Tian Q, Yin G, et al. Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed[J]. J Plant Physiology, 2014, 171(2): 140-147.
[3]董国军, 胡兴明, 曾大立, 等. 水稻种子人工老化和自然老化的比较研究[J]. 浙江农业科学, 2004(01): 27-29.(DONG Guojun, HU Xingming, ZENG Dali, et al.Comparative Study on Artificial and Natural Aging of Rice Seeds[J].Zhejiang Agricultural Science, 2004(01):27-29. )
[4]张瑛, 滕斌, 吴敬德, 等. 水稻种子高温高湿人工加速老化试验方法研究[J]. 中国粮油学报, 2010, 25(010): 8-12.(ZHANG Ying, TENG Bin, WU Jingde, et al. Study on Accelerated Aging Test of Rice Seeds with High Temperature and Humidity[J]. J Chinese Cereals and Oils Association, 2010, 25(010):8-12 )
[5]胡国玉, 张磊, 黄志平, 等. 大豆种子抗老化鉴定的方法研究[J]. 大豆科学, 2012, 31(003): 389-394.(HU Guoyu, ZHANG Lei, HUANG Zhiping, et al. Identification Method of Resistance to Seed Aging in soybean(Glycine max L. Merr)[J]. Soybean Science, 2012, 31(003):389-394.)
[6]Kamizake N K K, Yamashita F, Prudencio S H, et al. Physical alterations of soybean during accelerated and natural aging[J]. F Research International, 2014, 55: 55-61.
[7]高华伟, 满强, 潘晶, 等. 大豆人工老化与自然老化的种子活力差异研究[J]. 种子, 2015, 34(1): 14-18.(GAO Huawei, MAN Qiang, PAN Jing, et al.Differences in Properties of Seed Vigor Between Artificially and Naturally Aged Soybean Seeds[J]. Seed,2015,34(1)14-18.)
[8]Zaheer A, Hui Y, Yong-Bi F, et al. The Associative Changes in Scutellum Nuclear Content and Morphology with Viability Loss of Naturally Aged and Accelerated Aging Wheat (Triticum aestivum) Seeds[J]. Frontiers in Plant, 2016, 7: 1474.
[9]Zhang X, Xu M, Hina A, et al. Seed storability of summer-planting soybeans under natural and artificial aging conditions[J].Legume Research, 2018, 404: 1-10.
[10]高琴梅, 卢新雄, 朱凌燕, 等. 大豆种子老化MDA和4-HNE的含量变化相关性研究[J]. 种子, 2019, 38(04):1-9. (GAO Qinmei, LU Xinxiong, ZHU Lingyan, et al.Correlation Studies on MDA and 4-HNE Contents in Soybean Seed Aging[J]. Seed, 2019, 38(04):1-9.)
[11]Xu L, Zhu X, Chen X, et al. Direct FTIR analysis of isolated trans fatty acids in edible oils using disposable polyethylene film[J]. Food Chemistry, 2015, 185: 503-508.
[12]Guo X X, Hu W, Liu Y, et al. Rapid determination and chemical change tracking of benzoyl peroxide in wheat flour by multi-step IR macro-fingerprinting[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2016, 154: 123-129.
[13]Liu Y, Hu W, Guo X X, et al. Rapid discrimination of three marine fish surimi by Tri-step infrared spectroscopy combined with Principle Component Regression[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, 149: 516-522.
[14]杨卫梅, 刘刚, 欧全宏,等. 豆类种子自然老化过程的红外光谱研究[J]. 激光与光电子学进展, 2018, 55(012): 5123001.(YANG Weimei, LIU Gang, OU Quanhong, et al. Infrared Spectroscopy of Naturally Aged Legume Seeds[J]. Laser & Optoelectronics Progress, 2018, 55(012): 123001.)
[15]Zhao X, Chen F, Xue W, et al. FTIR spectra studies on the secondary structures of 7S and 11S globulins from soybean proteins using AOT reverse micellar extraction[J]. Food Hydrocolloids, 2008, 22(4): 568-575.
[16]Wang C, Jiang L Z, Wei D X, et al. Effect of Secondary Structure determined by FTIR Spectra on Surface Hydrophobicity of Soybean Protein Isolate[J]. Procedia Engineering, 2011, 15(1): 4819-4827.
[17]Gönül A, Şükriye N K E, Fatma N A, et al. Chemometric classification and quantification of cold pressed grape seed oil in blends with refined soybean oils using attenuated total reflectance-mid infrared (ATR-MIR) spectroscopy[J]. LWT, 2019, 100: 126-137.
[18]Yasar S, Tosun R, Snmez Z, et al. Fungal fermentation inducing improved nutritional qualities associated with altered secondary protein structure of soybean meal determined by FTIR spectroscopy[J]. Measurement, 2020, 161: 107895.
[19]Borisz C, Peinar I, Petrovi I, et al. Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization[J]. Journal Cereal Science, 2019, 87: 25-30.
[20]张自阳, 姜小苓, 茹振钢, 等. 人工老化对杂交小麦种子生理特性和种子活力变化的影响[J]. 江苏农业科学, 2013, 041(002):81-83. (ZHANG Ziyang, JIANG Xiaoling, RU Zhengang, et al. Effects of artificial aging on physiological characteristics and vigor of hybrid wheat seeds[J]. Jiangsu Agricultural Sciences, 2013, 041(002):81-83.)

基金

国家自然科学基金项目(31760341)和云南省高校科技创新团队支持计划项目资助
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