本文提出一种基于多孔氧化铝(Anodic Aluminum Oxide,AAO)基板为模版，采用交流电场组装技术 在AAO 纳米孔内组装被十六烷基三甲基溴化铵(Cetyl-trimethylammonium bromide,CTAB)包覆的大金球 纳米颗粒，分析交流电场频率、电压对组装结果的作用影响，得到在孔内组装单颗粒的最佳制备条件。在此基 础上，将前述制备衬底浸泡NDT 分子溶液进行处理后，再将其浸泡在柠檬酸钠修饰的小金球胶体溶液中，小 金球与1,9-壬二硫醇(1,9-nonanedithiol,NDT) 分子修饰后的大金球嫁接组装，得到异构二聚体@AAO 阵 列衬底。选用罗丹明6G、结晶紫、阿斯巴甜为探针分子，探究所衬底具有高拉曼活性和增强均匀性，所得结果 为拓展拉曼光谱技术的工程应用提供实验支撑。

In this paper,we propose a method based on porous alumina(AAO)substrate as a template,using Alternating current(AC)electric field assembly technology to assemble large gold ball nanoparticles coated with CTAB in AAO nanopores,and analyze the effects of AC electric field frequency and voltage on the assembly results,and obtain the optimal conditions for the preparation of self-assembled single particles in the pores.On this basis,the prepared substrate was soaked in a colloidal solution of small gold balls modified with sodium citrate, and the single particles modified by NDT molecules were quantitatively coupled with a single morphology and coated with different nanoparticles on the surface to obtain Heterodimers@ AAO array substrate.Rhodamine 6G,Crystal violet and Aspartame were selected as probe molecules to explore the high Raman activity and enhanced uniformity of the substrates,and the obtained results provided experimental support for the engineering application of Raman spectroscopy.