The narrow-band thin layer chromatography combing with surface enhanced Raman spectroscopy (narrow-band TLC-SERS) method was established to further improve the sensitivity of conventional TLC-SERS method, which can restrain transverse diffusion of analytes in TLC development and overcame the second diffusion of analytes attributed to adding SERS-active substrate. In this work, the narrow-band TLC plate with width of 2 mm was prepared using the silica gel GF254 as stationary phase. After the sample applied on the narrow-band TLC plate, the silver colloid was deposited on the surface of separated spots, and then the spots were detected by portable Raman spectrometer. The results indicate that the narrow-band TLC-SERS method is simple, rapid and sensitive. Furthermore, this method not only restrains the defect of the transverse diffusion of analytes in TLC development but also saving the cost. Thus, the narrow-band TLC-SERS method has good prospects for application.