The solid phase glucose structure had been studied by fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR). The OH stretch vibration (νOH), CH2 asymmetrical stretching vibration (νasCH2), CH2 symmetrical stretching vibration (νsCH2) , CH2 scissoring vibration (δCH2), C-O stretch vibration (νC-O), O-H in-plane bending vibration (δOH), glucose skeleton vibration mode（νⅠ) and glucose skeleton breathing vibration(νⅢ) were found. The solid phase glucoses νⅠ and νⅢ had been discussed by two-dimensional infrared spectroscopy (2D-IR). The α-D-glucopyranose and β-D-glucopyranose were discoverd and influence of temperature change on solid glucose structure was researched by 2D-IR also.