The surface-enhanced Raman spectroscopy (SERS) method with internal standard was established to determine graphite in water-borne organic paints. The working samples, which were tested by Raman spectroscopy, were prepared by adding graphite into poly(butyl acrylate) latex substrate, followed by pipeting potassium thiocyanate (KSCN) as internal standard and silver nano-particles (Ag NP). Since 1584 cm^-1 was recognized as graphite’s G peak while 2124 cm^-1 as KSCN’s peak, standard curve was made by calculating the ratio of the two peaks, which exhibited good linearity with r²=0.9994 and 0.9934 for 5000 mesh graphite and 200 mesh graphite, respectively. For 5000 mesh graphite, the limit of detection (LOD) is 0.12%, and precision was 6.99%. The spiked recovery of poly(butyl acrylate) and polyvinyl acetate substrates was 79.49% and 76.29%, respectively. All the indices met the testing requirements. This method was convenient, rapid and accurate, and could be used as the quality control method for water-borne organic paints with carbon materials.