Identification of Fungal Spores of Puffball Species in Medicinal Fungi by Fourier Transform Infrared Spectroscopy
Author information+
{{custom_zuoZheDiZhi}}
{{custom_authorNodes}}
{{custom_bio.content}}
{{custom_bio.content}}
Collapse
History+
Published
2018-03-30
Issue Date
2018-04-02
Abstract
The purpose of this study was to identify medicinal fungi ofCalvatialilacina (Mont. Et Berk) Lloyd ,Calvatiagigantea (Batsch ex Pers.) Lloyd and Lasiosphaerafenzlii Reich.which had similar morphological characteristics. Fourier transform infrared spectrometer was employed to obtain the spectra of spores of this three puffball species, and further researched their characteristics of molecular structure by spectral information. The infrared spectra of three puffball species demonstrated the absorption band of protein, polysaccharides and esters. The intensity and shape of these bands had differences between different medicinal fungi. The technique of Fourier Self-Deconvolution and fourth derivative spectrum combined with curve-fitting analysis was used to uncover the spectral information at fingerprint region of infrared spectra. Curve-fitting analysis of 1200~890 cm-1 presented the structure of polysaccharides had significant difference in three puffball species. The multi-component peaks at 1072 cm-1 and 1040 cm-1 corresponded to C-O and C-C stretching vibrations of polysaccharides, there were obvious differences between Calvatialilacina (Mont. Et Berk) Lloyd , Calvatiagigantea (Batsch ex Pers.) Lloyd and Lasiosphaerafenzlii Reich. The characteristic absorption peaks at 943 cm-1 and 892 cm-1 of Calvatialilacina (Mont. Et Berk) Lloyd indicated that it contain β-glycoside linkage, and the peaks at 920 cm-1 showed that α-glycoside linkage in Lasiosphaerafenzlii Reich.. The results suggested that Fourier transform infrared spectroscopy could provide the information of molecular structure of puffball fungus. It was a promising technique to identify medicinal fungi.
.
Identification of Fungal Spores of Puffball Species in Medicinal Fungi by Fourier Transform Infrared Spectroscopy. Chinese Journal of Light Scattering. 2018, 30(1): 56 https://doi.org/10.13883/j.issn1004-5929.201801011
