Surface-enhanced Raman scattering (SERS) can paves the way for the trace detection of low-concentration analyses, even single molecular detection, and owns greatly potential application prospect in chemistry, biology, environment, and so on. Development of high-performance SERS substrate is one of the most import research topic in SERS technology. In this work, we achieve the fabrication, characterization, and SERS effect research of a kind of high-performance, low-cost, and large area SERS substrate. The Raman scattering intensity from R6G molecule can be effectively enhanced by this SERS substrate for about five times when compared with that with roughness Au film. We carry out systematic numerical simulation and experiment research on the influence of micro-sphere diameter and nano-particle height on the Raman scattering enhanced factor, and find an optimized structure parameters. Our work will provide a kind of high-performance SERS substrate.