Study on Quantitative Measurement Methods of Biological Organization Based on Raman Spectroscopy

doi:10.13883/j.issn1004-5929.201903008

Chinese Journal of Light Scattering ›› 2019, Vol. 31 ›› Issue (3) : 260-265. DOI: 10.13883/j.issn1004-5929.201903008

Applications in Chemistry and Biology Researches

Study on Quantitative Measurement Methods of Biological Organization Based on Raman Spectroscopy

ZHU Yongkang, LU Yanfei, SHANG Linwei, ZHAO Yuan, JIANG Chongyu, XU Hao, YIN Jianhua^*

Author information +

History +

Abstract

The Raman intensity of different biological samples or slices at the same wavenumber would be probably different when repeatedly detected at the same location,which was due to fine variation of focused position and the system deviation and made quantitative analysis much hard.Consequently,it was necessary to get a more feasible approach of defining internal or external standard for quantitative detection,which would be helpful for comparing Raman spectra captured under different conditions directly.Under the condition of polarization and depolarization,the Raman spectra of canine hip articular cartilage slices were detected and processed by standard normal variate (SNV) and multi-scattering correction (MSC) methods.In addition,eosin used as a novel internal standard to preprocess Raman signal was discussed and explored.It was found that the MSC method was most suited for the depolarization condition,as well as 1501 cm^-1 band of eosin as internal standard for the polarization condition.The Raman band of eosin didn′t show anisotropy with polarization angel,which was effective to use as internal standard for the normalization of Raman spectra of biological tissue.Further,the internal standard method based on eosin brought more simple operation and more accurate results than MSC and SNV.Therefore,this study provided a promising novel method for the quantitative analysis of biological samples based on Raman spectroscopy.

Key words

Quantitative analysis / Raman spectroscopy / Eosin / MSC / SNV / Cartilage

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHU Yongkang, LU Yanfei, SHANG Linwei, ZHAO Yuan, JIANG Chongyu, XU Hao, YIN Jianhua. Study on Quantitative Measurement Methods of Biological Organization Based on Raman Spectroscopy. Chinese Journal of Light Scattering. 2019, 31(3): 260-265 https://doi.org/10.13883/j.issn1004-5929.201903008

References

[1] Vo-Dinh T,Yan F,Wabuyele M B.Surface-enhanced Raman scattering for biomedical diagnostics and molecular imaging.Surface-Enhanced Raman Scattering:Physics and Applications 2006,103,409-426.
[2] 周殿凤,柯惟中,衡航等. 紫外辐射对牛血清白蛋白影响的拉曼光谱分析[J].光散射学报,2006,18 (3) :241-247 (ZHOU Dianfeng,KE Weizhong,HENG Hang et al.Raman spectroscopic study on the influence of UV radiation on BSA[J].The Journal of Light Scattering,2006,18(3):241-247)
[3] Kneipp J,Witting B,Bohr H,et al.Surface-enhanced Raman scattering:a new optical probe in molecular biophysics and biomedicine.Theoretical Chemistry Accounts 2010,125(3-6),319-327.
[4] 褚小立,袁洪福,陆婉珍.近红外分析中光谱预处理及波长选择方法进展与应用[J].化学进展,2004,16(4):528.
[5] Seasholtz,Mary Beth,Kowalski B.The parsimoy principle applied to multivariate calibration.Analytica Chimica Acta 277.2(1993):165-177.
[6] Gao Hao,Wang Xiao,Shang L W,et al.Design and Application of Small NIR-Raman Spectrometer Based on Dichroic and Transmission Collimating.SPECTROSCOPY AND SPECTRAL ANALYSIS,2018,38(06):1933-1937.
[7] Gao Hao,Zhai M Y,Shang L W,et al.Articular Cartilage Optical Clearing Research by Raman Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,2018,38(08):2425-2429.
[8] VMclean D I,Zeng H,Lui H,et al.Toward instrument-independent quantitative measurement of fluorescence intensity in fiber-optic spectrometer systems[J].Applied Optics,2007,46(29):7132-7140.
[9] Isaksson T,Naes T.The Effect of Multiplicative Scatter Correction (MSC) and Linearity Improvement in NIR Spectroscopy[J].Applied Spectroscopy,1988,42(7):1273-1284.
[10] Chen J,Iyo C,Terada F,et al.Effect of multiplicative scatter correction on wavelength selection for near infrared calibration to determine fat content in raw milk[J].J Near Infrared Spectrosc,2002,10(1):301-307.
[11] 芦永军,曲艳玲,宋敏.多元散射校正算法用于近红外相关光谱的处理研究[C].全国近红外光谱学术会议.2006.
[12] Wen T C,Ming T L,Han N L,et al.Micro-Raman spectroscopy used to identify and grade human skin pilomatrixoma[J].Microscopy research technique,2005,64(2):75-79.
[13] Eoghan ó Faoláin,Mary B,Hunterb,Joe M.Byrne,et al.A study examining the effects of tissue processing on human tissue sections using vibrational spectroscopy[J].Vibrational Spectroscopy,2005,38(1-2):121-127.
[14] Richard Ellis,Ellen Green,C.Peter Winlove.Structural Analysis of Glycosaminoglycans and Proteoglycans by Means of Raman Microspectrometry [J].Connective Tissue Research,2009,50(1):29-36.
[15] 吴正洁,黄耀熊,王成,等.多种拉曼光谱归一化法对乙醇定量分析的研究[J].光谱学与光谱分析,2010,30(4):971-974.
[16] 李梁,邵磊,霍剑青等.拉曼光谱退偏度测量实验的设计及其应用的研究[J].物理与工程,2008,18(3):65-69.
[17] Kazanci M,Roschger P,Paschalis E P,et al.Bone osteonal tissues by Raman spectral mapping:Orientation-composition[J].Journal of Structural Biology,2006,156(3):489-496.
[18] Kazanci M,Wagner H D,Manjubala N I,et al.Raman imaging of two orthogonal planes within cortical bone[J].Bone,2007,41(3):456-461.
[19] Raghavan M,Sahar N D,Wilson R H,et al.Quantitative polarized Raman spectroscopy in highly turbid bone tissue.[J].Journal of Biomedical Optics,2010,15(3):037001.

PDF(1139 KB)

115

Accesses

Citation

Detail

Sections

Recommended

Abstract
Key words
Cite this article
References

Received	Revised	Published
2018-09-05	2018-09-15	2019-09-10
Issue Date
2022-08-30

Please choose a citation manager

Content to export

Abstract

Key words

Cite this article

{{custom_sec.title}}

{{custom_sec.title}}

References

{{custom_fnGroup.title_en}}

Footnotes