Detection of 6-Meraptopurine in urine by Surface enhanced Raman spectroscopy

WANG Yongtao, HUANG Guangyao, Li Pan, WANG Hongzhi, YANG Liangbao

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Chinese Journal of Light Scattering ›› 2019, Vol. 31 ›› Issue (4) : 361-366. DOI: 10.13883/j.issn1004-5929.201904008
Application in Chemistry and Biology Researches

Detection of 6-Meraptopurine in urine by Surface enhanced Raman spectroscopy

  • WANG Yongtao1,2, HUANG Guangyao1,2, Li Pan1, WANG Hongzhi1,3*, YANG Liangbao1*
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Abstract

This paper uses the Surface enhanced Raman spectroscopy (SERS) to achieve the sensitive detection of the anti-tumor drug 6-Meraptopurine (6-MP). Using the seed growth method, the diameter of the synthesized nanoparticles (PVP-AuNPs) is 62-68 nm. The PVP-AuNPs demonstrated the uniformity and monodisperse collected from the Scanning Electron Microscopy (SEM) and UV-vis absorption spectra. PVP-AuNPs as SERS active substrates realize the sensitive detection of 6-MP with level of 10-6 mol/L. After the appropriate pretreatment, the test limitation for 6-MP in the urine can reach 10-4 mol/L.

Key words

Anti-tumor drugs / 6-mercaptopurine / Gold nanoparticles / Surface enhancement Raman spectroscopy / urine pretreatment

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WANG Yongtao, HUANG Guangyao, Li Pan, WANG Hongzhi, YANG Liangbao. Detection of 6-Meraptopurine in urine by Surface enhanced Raman spectroscopy. Chinese Journal of Light Scattering. 2019, 31(4): 361-366 https://doi.org/10.13883/j.issn1004-5929.201904008

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