Detection of Alkaline Phosphatase Activity by using Surface-Enhanced Raman Spectroscopy

JIANG Lei, GAN Zhenfei, CHEN Huaying, CHANG Shuai, LI Jiabin, LI Dawei

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Chinese Journal of Light Scattering ›› 2019, Vol. 31 ›› Issue (4) : 367-372. DOI: 10.13883/j.issn1004-5929.201904009
Application in Chemistry and Biology Researches

Detection of Alkaline Phosphatase Activity by using Surface-Enhanced Raman Spectroscopy

  • JIANG Lei, GAN Zhenfei, CHEN Huaying, CHANG Shuai, LI Jiabin, LI Dawei*
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Abstract

Alkaline phosphatase (ALP) is a critical enzyme biomarker in living organisms, its abnormal expression has close relationship with liver dysfunction, bone disease and so on. In this work, a novel method based on surface enhanced Raman spectroscopy (SERS) is proposed to detect ALP activity by developing a probe bis-s-s’-(3-mercaptophenyl disodium phosphate) (B-MDP). The detection can be realized by the significant differences in SERS spectra between the probe and its hydrolysis product which is triggered by ALP. Owing to the comprehensive superiority integrating the specificity of catalytical action with the fingerprinting feature of SERS technique, the SERS probe shows high selectivity towards ALP. In addition, the proposed approach demonstrates good linear relationships in the range of 0.01-0.5 mU/mL and 0.5-10 mU/mL with low limit of detection at 3 μU/mL (S/N=3). Furthermore, the proposed SERS method performs well on detecting ALP in serum and evaluating ALP inhibitor, thereby showing a great promising for the early diagnosis and drug screening of ALP-associated diseases.

Key words

surface-enhanced Raman scattering / alkaline phosphatase / probe / activity detection / serum sample

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JIANG Lei, GAN Zhenfei, CHEN Huaying, CHANG Shuai, LI Jiabin, LI Dawei. Detection of Alkaline Phosphatase Activity by using Surface-Enhanced Raman Spectroscopy. Chinese Journal of Light Scattering. 2019, 31(4): 367-372 https://doi.org/10.13883/j.issn1004-5929.201904009

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