Ultra-sensitive Detection of Leukemia Fusion Gene (b3a2) by SERS Spectra

WANG Yunyi, LIN Xueliang, XIE Jiawen, QIN Tiandi, HUANG Yun, HUANG Huifang, FENG Shangyuan

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Chinese Journal of Light Scattering ›› 2019, Vol. 31 ›› Issue (4) : 373-380. DOI: 10.13883/j.issn1004-5929.201904010
Application in Chemistry and Biology Researches

Ultra-sensitive Detection of Leukemia Fusion Gene (b3a2) by SERS Spectra

  • WANG Yunyi1, LIN Xueliang1, XIE Jiawen1, QIN Tiandi2, HUANG Yun2, HUANG Huifang2*, FENG Shangyuan1*
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Abstract

In patients with Chronic Myeloid Leukemia (CML), fusion genes appear and produce corresponding proteins, which have kinase functions that severely affect cell metabolism, leading to abnormal cell cycle and eventually deterioration into cancer cells. The BCR-ABL fusion gene (b3a2) is clinically useful as a diagnostic marker for chronic myeloid leukemia, but current clinical detection methods have defects such as low sensitivity and specificity. In this study, a silane coupling agent was used to modify the silica sheet and protonated to adsorb the negatively charged silver nanoparticles (AgNPs). Finally, the silver and amino groups were firmly bonded to the silica sheet to form a two-dimensional Surface-Enhanced Raman Spectroscopy (SERS) substrate. Then, the BCR-ABL fusion gene (b3a2) was quantitatively detected by detecting the SERS signal of Cy5 by combining the signal chain modified with positive AgNPs and the capture chain modified on SERS substrate with the BCR-ABL fusion gene (b3a2). The results show that the BCR-ABL fusion gene (b3a2) could be detected with high sensitivity and specificity, and the detection limit could reach 42.28 fmol / L. This method provides a potential solution for clinically ultrasensitive detection of BCR-ABL fusion gene, and can well solve the problems of low sensitivity and weak specificity in clinical detection.

Key words

Chronic myeloid leukemia (CML) / BCR-ABL fusion gene (b3a2) / Surface-enhanced Raman spectroscopy (SERS) / Ultra-sensitive detection

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WANG Yunyi, LIN Xueliang, XIE Jiawen, QIN Tiandi, HUANG Yun, HUANG Huifang, FENG Shangyuan. Ultra-sensitive Detection of Leukemia Fusion Gene (b3a2) by SERS Spectra. Chinese Journal of Light Scattering. 2019, 31(4): 373-380 https://doi.org/10.13883/j.issn1004-5929.201904010

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