Rose-shaped MoS₂ nanoparticles (NPs) were synthesized by hydrothermal method, and the MoS₂@Au nano-composites (NCs) were prepared by oxidation-reduction method, which were used as the surface enhanced Raman scattering (SERS) active substrates. Scanning electron microscope, transmission electron microscope, X-ray diffractometer and Raman spectrometer were used to characterize the structure performance of MoS₂@Au NCs. We applied Rhodamine 6G (R6G) as the probe molecules, the SERS properties of the MoS₂ NPs and MoS₂@Au NCs were studied. The results show that under the excitation of 532 nm laser, the detection concentration limit of R6G is 10^-6 M when MoS₂ NPs is used as the substrate. After the modification of Au NPs, the detection limit can be reached 10^-9 M. It is obviously improved by 3 orders of magnitude, which is due to the enhancement of electromagnetic field of Au nanoparticles and the enhancement of charge transfer between substrate and molecule to promote the improvement of SERS performance. Furthermore, the relative standard deviation (RSD) of the SERS signal of MoS₂@Au substrate was calculated to be 12.2%, this indicates that the SERS signal of the MoS₂@Au substrate has good uniformity.