Famille-rose is one of the four famous types of porcelain in Jingdezhen. Its characteristics of soft powder and rich color are closely related to the nature of the raw materials used. Scientific detection of famille-rose porcelain can provide a basis for studying the inheritance and development of famille-rose porcelain. Three-dimensional video microscope, X-ray fluorescence scanning Imager (MA-XRF) and Raman spectrometer were used for in situ non-destructive testing of Yongzheng famille-rose bottles in the collection. It was found that the coloring agent of purple color was gold red, the coloring agent of red color was alum red, the coloring agent of green color was copper compound, the coloring agent of yellow color was lead-tin yellow type II, and the yellow-green color was made of green color and yellow color. The black colorant is manganese oxide. The combination of microscope and MA-XRF can reveal the relationship between the upper and lower layers of the pigment, the painting technique of the famille-rose, the characteristic elements of the pigment and its distribution position. The application of three kinds of in-situ nondestructive testing instruments can provide reference for the scientific research of the contents of complete porcelain colorant and painting techniques.