Surface-enhanced Raman Scattering (SERS) is an analytical method based on molecular vibration fingerprint information, which has high sensitivity, good selectivity, nondestructive and on-site detection, and is free from the interference of water system. It has potential application in the fields of biological analysis and early diagnosis. Compared with natural enzyme, nanoenzyme as an artificial mimic enzyme presents the advantages of easy and scalable preparation, diversity of enzymatic activity via rational design as well as long-term environmental stability. Metal-organic framework (MOF) with mesoporous crystal structure, large specific surface area could be easily synthesized, which is beneficial to immobilization of other nanomaterials to improve stability and realize the multifunctional performance. This short review concerns on nano-enzyme, MOF and SERS substrate assembly to advance the figure of analytical merit, and focuses on the current explorations of MOF/nanozyme/SERS platform in the field of biomedical analysis and prospects.