Supported planar lipid bilayers are artificial lipid bilayer membranes. Surface structures and properties of the solid substrates can affect the formation process, fluidity, two-dimensional structure and chemical activity of the SPBs, then affect properties of protein. Even on mica and SiO2 surfaces, which are flat and biologically inert, and most widely used as the substrates for the supported lipid bilayers, cause differences in the structure and properties of the supported membranes. In this review, by performing Raman spectroscopy, we analyzed the conformation of SPBs at mica and SiO₂. Futhermore, we added the monomer amyloid-beta peptides onto SPBs. The differences between the conformation of amyloid-beta at mica and SiO₂ are shown by Raman spectroscopy with different incubation time.