Methods for rapid inspection of illegally added medicine in anti-fatigue health food using Raman spectroscopy is investigated. Raman scattering, superposition of Raman scattering and surface enhanced Raman scattering are combined to get specific Raman signal of samples, and the illegally added medicine can be inspected by comparing the Raman spectra of the samples with those of medicine. Hundreds of samples were inspected and the accuracy is higher than 95 percent, which indicates the method available for the inspection of illegally added medicine in anti-fatigue health food.