10 March 2020, Volume 32 Issue 1
    

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    Overview
  • XU Di, HUANG Qing
    Chinese Journal of Light Scattering. 2020, 32(1): 1-22. https://doi.org/10.13883/j.issn1004-5929.202001001
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    Microfluidic chips have attracted a lot of attention for their precise manipulation of micro-volume samples. Surface-enhanced Raman spectroscopy (SERS) as an ultra-sensitive analytical technique has been widely applied in trace detection in recent years. The combination of microfluidic chip and SERS can provide rapid, non-destructive, high-sensitivity and high-throughput analysis for biological samples, which is of great significance to the development of potential biomedical applications, thus occupying an outstanding position among the current research hot topics. This paper briefly introduces the recent developments of microfluidic SERS systems, including the fabrication of microfluidic chips, the manipulation of fluid and the integration of SERS substrates in microfluidic chips, and in particular attempts to give a comprehensive review on the biomedical applications of microfluidic SERS systems in recent years, including biomolecule detection, cell analysis, drug monitoring and screening, disease diagnosis, environment/food health and safety inspection.
  • LIU Yingxia, JIA Shuoguo
    Chinese Journal of Light Scattering. 2020, 32(1): 23-29. https://doi.org/10.13883/j.issn1004-5929.202001002
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    Raman spectroscopy is widely applied in forensic sciences especially in the identification of inkblot because of its trace, non-destructivness and efficient. The common Raman spectroscopy and the progress of inkblot identification have been summarized in this paper. And establishing a standard database of ink blot Raman spectra,combination of multiple detection technologies and chemometrics and other analytical methods are the development trends of Raman spectroscopy.
  • Theories of Light Scattering
  • DENG Rong, WANG Zhao, YANG Liang, LI Jin, YANG Zejin
    Chinese Journal of Light Scattering. 2020, 32(1): 30-39. https://doi.org/10.13883/j.issn1004-5929.202001003
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    To reveal the effects of different cage occupations on hydrate structure and related properties. The relaxation of the structure, electronic density of state and optical properties of the SI methane hydrates with three different configurations are calculated by first-principle, including (cI) only one large cage lacks its unique methane molecules; (cII), only one of the small cage lacks its unique methane molecule; (cIII) each cage is fully filled with methane molecules. The results show that the hydrate is most stable in the cIII due to its perfect structure, the cII is less stable, whereas the cI is least stable owing to its larger structural distortion after the loss of methane. On the contrary, loss of a methane molecule from cII causes negligible deformation. The relative change in cell volume is 0.56% and 2.1%, corresponding to the cII and the cI, respectively. The electronic density of states and the energy band gap of the cII is almost the same with those of cIII, differing obviously with those of cI. The calculation results display that the contribution of electronic transition is small, and proton disorder is the dominant hydrate permittivity. Methane hydrate is only responsive to light in the ultraviolet region, revealing their similar properties, regardless of their structural discrepancies, or/and the different ratios of water and methane molecules, 46/8=5.75 versus 46/7=6.57. Our calculations demonstrate that the lack of one methane in the cII causes negligible influence to the lattice structure and therefore to the electronic and optical properties in comparison with the cIII, whereas the lack of one methane in the cI can cause detectable changes. These results might provide valuable reference to the industrial exploration.
  • YAKUPU Xiayiding, TUERSUN Paerhatijiang, WU Panpan
    Chinese Journal of Light Scattering. 2020, 32(1): 40-45. https://doi.org/10.13883/j.issn1004-5929.202001004
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    For the application of gold nanoparticles in chemical and biological sensing, finding the shape of gold nanoparticles with high figure of merit has become a research hotspot in recent years. Based on the T-matrix method with the size-dependent dielectric function, the influence of the size of gold nanospheroids on its refractive index sensitivity, linewidth and figure of merit was studied quantitatively in this paper. In order to obtain the best sensing performance, the figure of merit was optimized and the optimal particle size parameters were obtained. The results show that the gold nanospheroids with the minor semi-axis of 11 nm and the major semi-axis of 49 nm has a maximum figure of merit of 6.76. The optimized gold nanospheroids can be treated as potential candidates for chemical and biological sensing. This study provides an important theoretical basis for the application of gold nanospheroids in chemical and biological sensing.
  • SurfaceEnhanced Raman Scattering(SERS)
  • TAO Chenyang, NING Huan, CHEN Haiyi, FAN Tianyu, ZHAN Yiting, YANG Shuo
    Chinese Journal of Light Scattering. 2020, 32(1): 46-51. https://doi.org/10.13883/j.issn1004-5929.202001005
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    Flower-like silver nanoparticles are well-controlled synthesized at room temperature by using silver nitrate as silver precursor, ascorbic acid as reductant and polyvinyl pyrrolidone with different concentrations as surfactant. Their structure and elemental composition are characterized by scanning electronic microscopy, atomic force microscope, X-ray diffraction and energy dispersive X-ray spectroscopy. When the concentration of polyvinyl pyrrolidone is up to 0.1 mol/L, the finest surface structure of the prepared flower-like silver nanoparticles is obtained, and the size of the particles reaches micron scale, which is suitable for the location and optical property study of single particles. The surface-enhanced Raman scattering (SERS) activity of individual and a few structure-optimized flower-like silver nanoparticle substrates are investigated using p-hydroxybenzoic acid as probes. The SERS mechanism of the substrates is analyzed by mean of dark field scattering spectroscopy. The experimental results indicate that the special surface structures of the flower-like silver nanoparticles provide large quantities of “hot spots” for the enhanced Raman intensity. The satisfying SERS properties combined with low synthesis costs show the prospective application possibilities of this novel SERS substrate.
  • PEI Yuan, WU Zhengang, WEI Hengyong, WU Mingming, CHEN Ying, WEI Yingna, LIU Zhanjun, LIU Yanmei, WANG Xuepei, LIU Haiyong
    Chinese Journal of Light Scattering. 2020, 32(1): 52-57. https://doi.org/10.13883/j.issn1004-5929.202001006
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    V2O5 gel was prepared with C10H14O5V as V source by non-hydrolytic sol-gel method,then the V2O5 films was reduction nitriding by ammonia gas.XRD,FE-SEM, UV-VIS-NIR and RAMAN were used to characterize the membrane structure, optical properties and SERS effect.The results showed that with the increase of nitridation temperature, the grain size on the film increased gradually, and the grain development became better and better, and the crystal size reached 25 nm.After 600 ℃ reduction get the thin films, UV visible near infrared region the highest absorption rate, and forbidden band width is smaller than that of V2O5 films without nitriding.R6G was used as the probe molecule to study the SERS effect of thin film, and the results showed that the thin films had significant Raman enhancement effect at the nitridation temperature of 600, which was higher than that of V2O5 thin film without nitridation, and the Raman signal peak strength reached 485 cps at 620 cm-1.
  • Application in Chemistry and Biology Researches
  • DING Qianqian, GU Tao, DONG Ronglu
    Chinese Journal of Light Scattering. 2020, 32(1): 58-62. https://doi.org/10.13883/j.issn1004-5929.202001007
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    In this paper, Fe3O4 nanoparticles were prepared by co-precipitation method. And then the spiny Au nanostructures were formed on the surface of Fe3O4 nanoparticles by sodium citrate and hydroquinone reduction of chloroauric acid. The Fe3O4@Au nanostars as Surface-enhanced Raman spectroscopy (SERS) substrate used for thiram pesticide and ediphenphos detection. Due to high-density Au nanotip, the Fe3O4@Au nanostars exhibited high-sensitivity SERS activity. Additionally, this SERS substrate based on Fe3O4@Au nanostars can be found to be recyclable in virtue of the magnetic properties of Fe3O4 nanoparticles.
  • BAI Guohua, LU Yilin, LIAN Yuanyuan
    Chinese Journal of Light Scattering. 2020, 32(1): 63-71. https://doi.org/10.13883/j.issn1004-5929.202001008
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    In handwriting identification, when a case involves forged or altered strokes and figures problems the appraiser usually needs to examine the crossing section of the suspected material to confirm whether there is a forgery in the document. In addition, the study on the sequence determination of the crossing line can help appraiser to estimate the thumbprint of the handwriting and the character of the writing sequence. And also, those identification methods have reference value for the sequential identification of crossed writing and seal stamps documents. This paper draws experience from the existing methods with the common features, such as continuity of strokes and convergence phenomenon, and tests several types of black pens by Raman spectroscopy. Through the design of experiment to compare and choose the best parameters of the instrument and software settings, including microscope lens, laser wavelength, laser output power, spectral range, single acquisition time, cumulative acquisition times, scanning step, get the best experimental parameters and acquisition method and comparison principle. The experimental results of Raman spectrum 3D imaging are discussed in classification, analysis whether the application of this method to determine the writing order of cross strokes is useful. When preponderance and unanimous heterochromia phenomenon can be observed between the “hills and valleys”, an accurate conclusion can be drawn. When the heterochromia phenomenon is obvious but not incongruent or the phenomenon is not obvious, it need to combine the characteristics of stroke continuity in the 3D model to get the correct conclusion, but the accuracy is greatly influenced by the data processing and the 3D model imaging effect. At the same time, the undetectable situation is summarized, that is, when the Raman spectrum information is unable to be obtained effectively or the spectral information those are homology cannot be distinguished, the method is invalid. Based on the analysis and summarize of the experimental result, it can be sure that the Raman spectroscopy 3D profilometry techniques can get relatively objective and accurate judgment on the determination of sequence of black pen ink crossings under certain conditions, and the conclusion is affected little by the appraiser’s subjective judgment. It is worth a more thorough and comprehensive study in order to promote the application.
  • HU Linshun, ZENG Qingguang, WEN Jinxiu, CHEN Zikun, ZHANG Guoxiong
    Chinese Journal of Light Scattering. 2020, 32(1): 72-77. https://doi.org/10.13883/j.issn1004-5929.202001009
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    In this work, X-ray fluorescence spectroscopy and micro-Raman spectroscopy were used to analyze the components and molecular structures of blue-and-white porcelain excavated from Huawanping site in Shangchuan Island, Jiangmen City, Guangdong Province. It shows that the characteristics of the porcelain body are low aluminum, high silicon and high potassium. The main component of reddish-brown pigment is hematite employed with the glaze-on-color technology. The main component of blue pigment was the mixed with domestic cobalt soil and imported cobalt ore by the under-glaze color technology. It can be deduced that the porcelain originated from Zhangzhou kiln in Fujian Province at the late Ming or early Qing dynasties by combination historical background of the site at that time with the calculation of F function.
  • LU Yanfei, ZHU Yongkang, ZHAO Yuan, XU Xinyu, YIN Jianhua
    Chinese Journal of Light Scattering. 2020, 32(1): 78-83. https://doi.org/10.13883/j.issn1004-5929.202001010
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    Fourier transform infrared spectroscopy (FTIR) imaging technique can be used to simultaneously obtain spatial distribution and infrared spectral information of samples. In this study, when in vitro breast tissue samples were investigated by using FTIR imaging, and the differences between healthy and cancerous breast tissues were compared by characteristic band absorbance ratio (area ratio) images combining with FTIR spectroscopy analysis. It was found that, compared with healthy tissues, the characteristic band absorbance ratios in cancerous tissues significantly increased in the case of A1087/A1455、A1238/A1455、A1550/A1455、A1650/A1455, and reduced in the case of A1160/A1087、A1740/A1550. The results demonstrated that the change rule of bio-macromolecule contain in carcinogenesis of breast tissue, which reflected the accuracy, visualization and feasibility of FTIR imaging technique in the diagnosis of breast cancer. Therefore, FTIR imaging has potential to become an accurate and effective technical means for basic research and clinical diagnosis.
  • Other Optical Spectroscopic Techniques and Applications
  • CHEN Zhikun, GUO Rui, CHENG Pengfei
    Chinese Journal of Light Scattering. 2020, 32(1): 84-89. https://doi.org/10.13883/j.issn1004-5929.202001011
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    Oil pollutants have the danger of destroying marine ecosystems and indirectly polluting the atmosphere and soil. It is of great significance to quickly and accurately detect the components and concentrations of pollutants. Due to the serious overlap of their spectra, it is difficult to distinguish them accurately by traditional fluorescence analysis. Based on laser-induced fluorescence technology, a fluorescent spectrum detection system was designed with xenon lamp as excitation light, and 0# diesel, 92# gasoline and kerosene were scanned and detected to obtain excitation/emission spectrum and optimal excitation/emission wavelength. The software algorithm part of the system is improved, and the Savitzky-Golay convolution smoothing is added to directly obtain a more accurate excitation/emission spectrum, which can more comprehensively and accurately reflect the fluorescence characteristic information of the substance. Compared with the spectrum obtained by the traditional fluorescence spectrometer, the experimental results verify the effectiveness of the fluorescence-induced fluorescence detection system of laser-induced fluorescence technology, and have higher sensitivity for the detection of fluorescence spectral signals of oil pollutants.
  • XIAO Peng, ZHANG Shujiao, LI Dongfeng, XIE Fei, LI Zhihong
    Chinese Journal of Light Scattering. 2020, 32(1): 90-94. https://doi.org/10.13883/j.issn1004-5929.202001012
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    Coal carbonization is one of important ways to use coal efficiently. Synchrotron radiation small angle X-ray scattering (SAXS) technology is an effective method to study the change of internal pore structure during the coal carbonization. In this paper, we used SAXS to characterize Yuwu (Shanxi) bituminous coal carbonization process (100~1200 ℃, the temperature difference between 100 °C) and analyzed the changes of fractal dimension and pore size distribution. The results indicated that the scattering image of samples was similar with each other, all of them presented isotropic characteristics and surface fractal structure during coal carbonization. The fractal dimension trended to increase on the whole. During the increase of coal carbonization temperature, the pore size distribution changed obviously. The results reflected that the structural characteristics of coal carbonization had different stages, which was of great significance for subsequent coal research.